The TFS Glacios Cryo-TEM is the only electron microscope at UC Davis which is capable of producing sub 3Å resolution 3D reconstructions in single particle cryo-EM. Equipped with X-FEG optics, a Gatan K3 direct electron detector, and automation software (SerialEM), it can capture from 1300 images/day (stage shift) up to 3000 images/day (beam image shift). An autoloader allows up to 12 grids at a time to be loaded into the microscope for high-throughput screening. A 10 Gb connection to our Franklin HPC cluster allows on-the-fly image processing for data quality assessment.