Facilities Statement for Proposals

The UC Davis BioEM Facility is located in Briggs Hall in the center of the UC Davis main campus. This facility contains a Thermo Fisher Scientific 200 keV Glacios electron microscope with a Gatan K3 direct electron detector and an autoloader and is used for grid screening and high-resolution data collection. The facility contains a PELCO easiGlow Glow Discharge Cleaning System, a Thermo Fisher Scientific Vitrobot Mark IV plunge freezing device, a Leica EM GP2 plunge freezing device, and a Denton Vacuum Bench Top Turbo III Carbon Evaporator for cryo-EM grid preparation. The Technical Director, Dr. Fei Guo, has extensive experience in grid optimization and high-resolution data collection for cryo-EM single-particle analysis. In addition to the necessary computational resources for operating the microscope, the Facility provides support for on-the-fly data processing and analysis. Grid screening and data collection are performed using SerialEM and SmartScope, and data processing for sample quality assessment can be performed using Warp and/or cryoSPARC.